Unparalleled Metabolism Research and Published Papers

by Paulo D’Amora 1,2,3,Ismael D. C. G. Silva 1,3,Steven S. Evans 1,2,Adam J. Nagourney 2,Katharine A. Kirby 4,Brett Herrmann 2,Daniela Cavalheiro 2,Federico R. Francisco 2,Paula J. Bernard 1,2 andRobert A. Nagourney 1,2,5,*

Barison GAS, D’Amora P, Izidoro MA, Corinti M, Martins LM, Bonduki CE, Castro RdeA, Girão MJBC, Gomes MTV. Metabolomic Profiling of Peripheral Plasma by GC-MS and Correlation With Size of Uterine Leiomyomas, Journal of the Endocrine Society 2022 May; 6(7):1-14, https://doi.org/10.1210/jendso/bvac061.

D'Amora P, Silva IDCG, Budib MA, Ayache R, Silva RMS, Silva FC, Appel RM, Júnior SS, Pontes HBD, Alvarenga AC, Arima EC, Martins WG, Silva NLF, Diaz RS, Salzgeber MB, Palma AM, Evans SS, Nagourney RA. Towards risk stratification and prediction of disease severity and mortality in COVID-19: Next generation metabolomics for the measurement of host response to COVID-19 infection. PLoS One. 2021 Dec 1;16(12):e0259909. doi: 10.1371/journal.pone.0259909. PMID: 34851990; PMCID: PMC8635335.

D'Amora P, Silva IDCG, Tewari KS, Bristow RE, Cappuccini F, Evans SS, Salzgeber MB, Addis-Bernard PJ, Palma AM, Marchioni DML, Carioca AAF, Penner KR, Alldredge J, Longoria T, Nagourney RA. Platinum resistance in gynecologic malignancies: Response, disease free and overall survival are predicted by biochemical signature: A metabolomic analysis. Gynecol Oncol. 2021 Aug 23:S0090-8258(21)00646-6. doi: 10.1016/j.ygyno.2021.08.001. Epub ahead of print. PMID: 34446269.

da Silva IDCG, de Castro Levatti EV, Pedroso AP, Marchioni DML, Carioca AAF, Colleoni GWB. Biochemical phenotyping of multiple myeloma patients at diagnosis reveals a disorder of mitochondrial complexes I and II and a Hartnup-like disturbance as underlying conditions, also influencing different stages of the disease. Sci Rep. 2020 Dec 14;10(1):21836. doi: 10.1038/s41598-020-75862-4.

Carioca AAF, Steluti J, Carvalho AM, Silva AM, Silva IDCGD, Fisberg RM, Marchioni DM. Plasma metabolomics are associated with metabolic syndrome: A targeted approach. Nutrition. 2020 Nov 20;83:111082. doi: 10.1016/j.nut.2020.111082.

da Silva I, da Costa Vieira R, Stella C, Loturco E, Carvalho AL, Veo C, Neto C, Silva SM, D'Amora P, Salzgeber M, Matos D, Silva CR, Oliveira JR, Rabelo I, Yamakawa P, Maciel R, Biscolla R, Chiamolera M, Fraietta R, Reis F, Mori M, Marchioni D, Carioca A, Maciel G, Tomioka R, Baracat E, Silva C, Granato C, Diaz R, Scarpellini B, Egle D, Fiegl H, Himmel I, Troi C, Nagourney R. Inborn-like errors of metabolism are determinants of breast cancer risk, clinical response and survival: a study of human biochemical individuality. Oncotarget. 2018 Aug 3;9(60):31664-31681. doi: 10.18632/oncotarget.25839.

Okada LSDRR, Oliveira CP, Stefano JT, Nogueira MA, Silva IDCGD, Cordeiro FB, Alves VAF, Torrinhas RS, Carrilho FJ, Puri P, Waitzberg DL. Omega-3 PUFA modulate lipogenesis, ER stress, and mitochondrial dysfunction markers in NASH - Proteomic and lipidomic insight. Clin Nutr. 2018 Oct;37(5):1474-1484. doi: 10.1016/j.clnu.2017.08.031.

Serafim PVP, Figueiredo AG Jr, Felipe AV, Turco EGL, Silva IDCGD, Forones NM. Study of lipid biomarkers of patients with polyps and colorectal cancer. Arq Gastroenterol. 2019 Oct-Dec;56(4):399-404. doi: 10.1590/S0004-2803.201900000-80.

Santos JR, Waitzberg DL, da Silva IDCG, Junior TCT, Barros LRC, Canuto GAB, Faccio AT, Yamaguchi LF, Kato MJ, Tavares MFM, Martinez AC, Logullo AF, Torrinhas RSMM, Ravacci G. Distinct pattern of one-carbon metabolism, a nutrient-sensitive pathway, in invasive breast cancer: A metabolomic study. Oncotarget. 2020 May 5;11(18):1637-1652. doi: 10.18632/oncotarget.27575.

The diagnosis of multiple sclerosis (MS) has changed over the last decade, but remains a composite of clinical assessment and magnetic resonance imaging to prove dissemination of lesions in time and space. The intrathecal synthesis of immunoglobulin may be a nonspecific marker and there are no plasma biomarkers that are useful in the diagnosis of MS, presenting additional challenges to their early detection.

In response to the rapid development of genetically engineered glyphosate-tolerant crops, the use of glyphosate-based herbicides (GBHs), in agriculture, has increased substantially. Currently, it is estimated that 747 million kg of GBHs are applied per year. Although several epidemiological studies have demonstrated that there are health risks associated with GBH exposure, the effects these chemicals have on the oxidative and inflammatory response in the brain are still unclear. In fact, alterations in these processes could contribute to the development of neurological diseases, such as Alzheimer’s disease and autism spectrum disorders. The present study exposed pregnant rats to GBH and evaluated changes in the expression of genes related to oxidnte defense and inflammation response and monitored the serum metabolome in the adult male offspring. Pregnant Wistar rats were administered distilled water or Roundup®, at either 5 and 50 mg/kg/day, (p.o.) from gestational day (GD) 18 to postnatal day (PND) 5. There was a significant increase in the gene expression levels of Neuroglobin (Ngb – oxygen storage and tissue protection) (105%, p = 0.031), Glutathione Peroxidase 1 (Gpx1 – oxidative stress) (95%, p = 0.005), Prostaglandin-Endoperoxidase Synthase 1 (Ptgs1 - inflammation) (109%, p = 0.033) and Hypoxia inducible factor 1 subunit alpha (Hif1α – oxygen sensor) (73%, p = 0.017), in the cerebellum of PND90 rats perinatally exposed to 50 mg GBH/kg/day. Moreover, both GBH-exposed groups displayed a significant decrease in the expression of Catalase (Cat – oxidative stress) (49%, p = 0.003; and 31% p = 0.050, respectively) expression, in the cortex. Serum metabolites analyses, from the same animals of each group, demonstrated that there were significant changes in the concentrations of lysophosphatidylcholine and phosphatidylcholine, which have been associated with neurodegenerative diseases. The results of the present study suggest GBH exposure during pregnancy alters the expression of genes associated with oxidant defense, inflammation and lipid metabolism. It is plausible that maternal GBH exposure could have lasting neuronal effects on the offspring later in life.